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Western blot(SDS PAGE) analysis of extracts from NCCIT cells.Using BBS10 Mouse mAb IgG [0EUJ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BBS10,C12orf58,BBS10_HUMAN,Bardet-Biedl syndrome 10 protein
UniProtID :Q8TAM1
MASS(da) :80,838
MW(kDa) :81kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human BBS10.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NCCIT cells
Function : Probable molecular chaperone that assists the folding of proteins upon ATP hydrolysis (PubMed:20080638). Plays a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). Involved in adipogenic differentiation (PubMed:19190184)..
Subcellular locationi :Cell projection, cilium.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.