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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using BTN3A3 Mouse mAb IgG [U2IK] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BTN3A3,BTF3,BT3A3_HUMAN,Butyrophilin subfamily 3 member A3
UniProtID :O00478
MASS(da) :65,002
MW(kDa) :65kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human BTN3A3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Plays a role in T-cell responses in the adaptive immune response..
Tissue specificity :Detected in peripheral blood mononuclear cells and in T-cells (at protein level). Detected in spleen and lymphocytes..
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.