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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Calpastatin Mouse mAb IgG [2908] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CAST,ICAL_HUMAN,Calpastatin
UniProtID :P20810
MASS(da) :76,573
MW(kDa) :115kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Calpastatin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.