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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CEP250,C-NAP1 Mouse mAb IgG [C84V] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CEP250,CEP2,CNAP1,CP250_HUMAN,Centrosome-associated protein CEP250
UniProtID :Q9BV73
MASS(da) :281,137
MW(kDa) :280kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CEP250,C-NAP1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : May be involved in ciliogenesis (PubMed:28005958). Probably plays an important role in centrosome cohesion during interphase..
Tissue specificity :Ubiquitously and weakly expressed.
Subcellular locationi :Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, cilium basal body. Cell projection, cilium, photoreceptor outer segment. Photoreceptor inner segment.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.