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Western blot(SDS PAGE) analysis of extracts from HuvEc cells.Using eNOS Rabbit mAb [WC86] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NOS3,NOS3_HUMAN,Nitric oxide synthase, endothelial
UniProtID :P29474
MASS(da) :133,275
MW(kDa) :140 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human eNOS
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HuvEc cells
Function : Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.., [Isoform eNOS13C]: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Tissue specificity :Platelets, placenta, liver and kidney..
Subcellular locationi :Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.