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eNOS Rabbit mAb [WC86]Cat NO.: A37450

Western blot(SDS PAGE) analysis of extracts from HuvEc cells.Using eNOS Rabbit mAb [WC86] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NOS3,NOS3_HUMAN,Nitric oxide synthase, endothelial

UniProtID :P29474

MASS(da) :133,275

MW(kDa) :140 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human eNOS

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HuvEc cells

Function : Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.., [Isoform eNOS13C]: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Tissue specificity :Platelets, placenta, liver and kidney..

Subcellular locationi :Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.