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Western blot(SDS PAGE) analysis of extracts from JAR cells.Using DLK1 Mouse mAb IgG [8GRU] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DLK1,DLK,DLK1_HUMAN,Protein delta homolog 1 [Cleaved into: Fetal antigen 1 ]
UniProtID :P80370
MASS(da) :41,300
MW(kDa) :45kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human DLK1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :JAR cells
Function : May have a role in neuroendocrine differentiation.
Tissue specificity :Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
Subcellular locationi :Membrane,Single-pass type I membrane protein. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.