ERCC1 Rabbit mAb [AB33]Cat NO.: A92620

Protein names ：ERCC1,ERCC1_HUMAN,DNA excision repair protein ERCC-1

UniProtID ：P07992

MASS(da) ：32,562

MW(kDa) ：39 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human ERCC1

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells

Function ： [Isoform 1]: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.., [Isoform 2]: Not functional in the nucleotide excision repair pathway.., [Isoform 3]: Not functional in the nucleotide excision repair pathway.., [Isoform 4]: Not functional in the nucleotide excision repair pathway..

Subcellular locationi ：[Isoform 1]: Nucleus.,[Isoform 2]: Cytoplasm. Nucleus.,[Isoform 3]: Nucleus.,[Isoform 4]: Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.