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EIF1 Mouse mAb[J297]Cat NO.: A18133

Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using EIF1 Mouse mAb IgG [J297] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :EIF1,SUI1,EIF1_HUMAN,Eukaryotic translation initiation factor 1

UniProtID :P41567

MASS(da) :12,732

MW(kDa) :13kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human EIF1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HEK-293 cells

Function : Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.