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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using FOX2/RBM9 Mouse mAb IgG [M8XT] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RBFOX2,FOX2,HRNBP2,RBM9,RTA,RFOX2_HUMAN,RNA binding protein fox-1 homolog 2
UniProtID :O43251
MASS(da) :41,374
MW(kDa) :48kDa,50and60kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human FOX2/RBM9.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha..
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.