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Western blot(SDS PAGE) analysis of extracts from A549 cells.Using HNRNPF Mouse mAb IgG [UFA2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HNRNPF,HNRPF,HNRPF_HUMAN,Heterogeneous nuclear ribonucleoprotein F [Cleaved into: Heterogeneous nuclear ribonucleoprotein F, N-terminally processed]
UniProtID :P52597
MASS(da) :45,672
MW(kDa) :46kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HNRNPF.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells
Function : Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state..
Tissue specificity :Expressed ubiquitously.
Subcellular locationi :Nucleus, nucleoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.