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Western blot(SDS PAGE) analysis of extracts from human adrenal gland tissue.Using HSD3B2 Mouse mAb IgG [4B0I] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HSD3B2,HSDB3B,3BHS2_HUMAN,3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 [Includes: 3-beta-hydroxy-Delta-steroid dehydrogenase ,Steroid Delta-isomerase ]
UniProtID :P26439
MASS(da) :42,052
MW(kDa) :42kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HSD3B2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human adrenal gland tissue
Function : 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids..
Tissue specificity :Expressed in adrenal gland, testis and ovary.
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass membrane protein. Mitochondrion membrane,Single-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.