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Western blot(SDS PAGE) analysis of extracts from DU 145 cells.Using KLK11 Mouse mAb IgG [N286] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KLK11,PRSS20,TLSP,UNQ649/PRO1279,KLK11_HUMAN,Kallikrein-11 [Cleaved into: Kallikrein-11 inactive chain 1,Kallikrein-11 inactive chain 2]
UniProtID :Q9UBX7
MASS(da) :31,059
MW(kDa) :30kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human KLK11.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :DU 145 cells
Function : Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues..
Tissue specificity :Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level)..
Subcellular locationi :[Isoform 1]: Secreted.,[Isoform 2]: Golgi apparatus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.