* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from A431 cells.Using Tissue Factor/CD142 Rabbit mAb [4TOJ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :F3,TF_HUMAN,Tissue factor
UniProtID :P13726
MASS(da) :33,068
MW(kDa) :45-53 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Tissue Factor/CD142
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cells
Function : Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade..
Tissue specificity :Lung, placenta and pancreas..
Subcellular locationi :[Isoform 1]: Membrane,Single-pass type I membrane protein.,[Isoform 2]: Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.