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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using MRRF Mouse mAb IgG [EX51] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MRRF,RRFM_HUMAN,Ribosome-recycling factor, mitochondrial
UniProtID :Q96E11
MASS(da) :29,277
MW(kDa) :25kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MRRF.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity)..
Subcellular locationi :Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.