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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using PYCR1 Mouse mAb IgG [US93] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PYCR1,P5CR1_HUMAN,Pyrroline-5-carboxylate reductase 1, mitochondrial
UniProtID :P32322
MASS(da) :33,361
MW(kDa) :33kDa,35kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PYCR1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress..
Subcellular locationi :Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.