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RASEF Mouse mAb[XSFA]Cat NO.: A37874

Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using RASEF Mouse mAb IgG [XSFA] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :RASEF,RAB45,RASEF_HUMAN,Ras and EF-hand domain-containing protein

UniProtID :Q8IZ41

MASS(da) :82,879

MW(kDa) :90kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RASEF.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :LNCaP cells

Function : Binds predominantly GDP, and also GTP (PubMed:17448446). Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules (PubMed:30814157)..

Tissue specificity :Down-regulated in cutaneous melanoma cells but not in breast cancer cells..

Subcellular locationi :Cytoplasm, perinuclear region.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.