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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using RASEF Mouse mAb IgG [XSFA] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RASEF,RAB45,RASEF_HUMAN,Ras and EF-hand domain-containing protein
UniProtID :Q8IZ41
MASS(da) :82,879
MW(kDa) :90kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RASEF.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : Binds predominantly GDP, and also GTP (PubMed:17448446). Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules (PubMed:30814157)..
Tissue specificity :Down-regulated in cutaneous melanoma cells but not in breast cancer cells..
Subcellular locationi :Cytoplasm, perinuclear region.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.