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Western blot(SDS PAGE) analysis of extracts from K-562 cells.Using RHOBTB3 Mouse mAb IgG [77HV] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RHOBTB3,KIAA0878,RHBT3_HUMAN,Rho-related BTB domain-containing protein 3
UniProtID :O94955
MASS(da) :69,413
MW(kDa) :69kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RHOBTB3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K-562 cells
Function : Rab9-regulated ATPase required for endosome to Golgi transport. Involved in transport vesicle docking at the Golgi complex, possibly by participating in release M6PRBP1/TIP47 from vesicles to permit their efficient docking and fusion at the Golgi. Specifically binds Rab9, but not other Rab proteins. Has low intrinsic ATPase activity due to autoinhibition, which is relieved by Rab9..
Tissue specificity :Ubiquitous. Highly expressed in neural and cardiac tissues, pancreas, placenta and testis..
Subcellular locationi :Golgi apparatus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.