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Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using Sam50 Mouse mAb IgG [Z9TB] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SAMM50,SAM50,CGI-51,TRG3,SAM50_HUMAN,Sorting and assembly machinery component 50 homolog
UniProtID :Q9Y512
MASS(da) :51,976
MW(kDa) :52kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Sam50.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells
Function : Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes (PubMed:22252321, PubMed:25781180). Required for the assembly of TOMM40 into the TOM complex (PubMed:15644312)..
Subcellular locationi :Mitochondrion outer membrane,Multi-pass membrane protein. Cytoplasm. Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.