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Sam50 Mouse mAb[Z9TB]Cat NO.: A27985

Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using Sam50 Mouse mAb IgG [Z9TB] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SAMM50,SAM50,CGI-51,TRG3,SAM50_HUMAN,Sorting and assembly machinery component 50 homolog

UniProtID :Q9Y512

MASS(da) :51,976

MW(kDa) :52kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Sam50.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :NIH/3T3 cells

Function : Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes (PubMed:22252321, PubMed:25781180). Required for the assembly of TOMM40 into the TOM complex (PubMed:15644312)..

Subcellular locationi :Mitochondrion outer membrane,Multi-pass membrane protein. Cytoplasm. Mitochondrion.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.