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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using STX3 Mouse mAb IgG [MRYW] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :STX3,STX3A,STX3_HUMAN,Syntaxin-3
UniProtID :Q13277
MASS(da) :33,155
MW(kDa) :35kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human STX3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : Potentially involved in docking of synaptic vesicles at presynaptic active zones. Apical receptor involved in membrane fusion of apical vesicles.., [Isoform B]: Essential for survival of retinal photoreceetors.., [Isoform 3]: Functions as a regulator of gene expression..
Tissue specificity :[Isoform A]: Expressed in small intestine, kidney, pancreas, placenta as well as in retina. Weaker expression in lung, liver and heart. Not expressed in brain and skeletal muscle..,[Isoform B]: Expressed only in the retina..,[Isoform 3]: Ubiquitously expressed..
Subcellular locationi :[Isoform A]: Apical cell membrane,Single-pass type IV membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.