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Western blot(SDS PAGE) analysis of extracts from A549 cells.Using TCF12 Mouse mAb IgG [1W36] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TCF12,BHLHB20,HEB,HTF4,HTF4_HUMAN,Transcription factor 12
UniProtID :Q99081
MASS(da) :72,965
MW(kDa) :80kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TCF12.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells
Function : Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').
Tissue specificity :Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.