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TGN46 Mouse mAb[X839]Cat NO.: A69162

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using TGN46 Mouse mAb IgG [X839] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TGOLN2,TGN46,TGN51,TGON2_HUMAN,Trans-Golgi network integral membrane protein 2

UniProtID :O43493

MASS(da) :45,880

MW(kDa) :90kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TGN46.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : May be involved in regulating membrane traffic to and from trans-Golgi network.

Tissue specificity :Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.

Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.