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TRAF5 Mouse mAb[0300]Cat NO.: A87677

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using TRAF5 Mouse mAb IgG [0300] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TRAF5,RNF84,TRAF5_HUMAN,TNF receptor-associated factor 5

UniProtID :O00463

MASS(da) :64,406

MW(kDa) :64kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TRAF5.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR..

Tissue specificity :Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood.

Subcellular locationi :Cytoplasm. Cytoplasm, cytosol.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.