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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using TRAF5 Mouse mAb IgG [0300] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TRAF5,RNF84,TRAF5_HUMAN,TNF receptor-associated factor 5
UniProtID :O00463
MASS(da) :64,406
MW(kDa) :64kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TRAF5.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR..
Tissue specificity :Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood.
Subcellular locationi :Cytoplasm. Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.