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Western blot(SDS PAGE) analysis of extracts from human colon tissue.Using transgelin/SM22 Mouse mAb IgG [NES3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TAGLN,SM22,WS3-10,TAGL_HUMAN,Transgelin
UniProtID :Q01995
MASS(da) :22,611
MW(kDa) :18+22kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human transgelin/SM22.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human colon tissue
Function : Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.