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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using TRIM45 Mouse mAb IgG [44QM] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TRIM45,RNF99,TRI45_HUMAN,Tripartite motif-containing protein 45
UniProtID :Q9H8W5
MASS(da) :64,359
MW(kDa) :64kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TRIM45.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway..
Tissue specificity :Expressed in skeletal muscle, brain, heart and pancreas..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.