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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using UBAC1 Mouse mAb IgG [VD4Y] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :UBAC1,GBDR1,KPC2,UBADC1,UBAC1_HUMAN,Ubiquitin-associated domain-containing protein 1
UniProtID :Q9BSL1
MASS(da) :45,338
MW(kDa) :46kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human UBAC1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle..
Tissue specificity :Ubiquitous..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.