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VAMP4 Mouse mAb[F7T3]Cat NO.: A55871

Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using VAMP4 Mouse mAb IgG [F7T3] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :VAMP4,VAMP4_HUMAN,Vesicle-associated membrane protein 4

UniProtID :O75379

MASS(da) :16,397

MW(kDa) :18kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human VAMP4.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HEK-293 cells

Function : Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.

Subcellular locationi :Golgi apparatus, trans-Golgi network membrane,Single-pass type IV membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.