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WFS1 Mouse mAb[5E6E]Cat NO.: A81030

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using WFS1 Mouse mAb IgG [5E6E] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :WFS1,WFS1_HUMAN,Wolframin

UniProtID :O76024

MASS(da) :100,292

MW(kDa) :100kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human WFS1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store (PubMed:16989814). Negatively regulates the ER stress response and positively regulates the stability of V-ATPase subunits ATP6V1A and ATP1B1 by preventing their degradation through an unknown proteasome-independent mechanism (PubMed:23035048)..

Tissue specificity :Highly expressed in heart followed by brain, placenta, lung and pancreas. Weakly expressed in liver, kidney and skeletal muscle. Also expressed in islet and beta-cell insulinoma cell line.

Subcellular locationi :Endoplasmic reticulum membrane,Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.