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Western blot(SDS PAGE) analysis of extracts from Recombinant SARS-COV-2 N Protein.Using SARS-CoV-2 N Protein Mouse mAb [VM79] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SARS-CoV-2 N Protein
UniProtID :P59595
MASS(da) :46,025
MW(kDa) : 50KDa
Form :Liquid
Purification :Protein A purification
Host :Mouse monoclonal IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :SARS-CoV-2
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of SARS-CoV-2 N Protein.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Recombinant SARS-COV-2 N Protein
Function :Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed:17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed:18055455).
Subcellular locationi :Virion,Host endoplasmic reticulum-Golgi intermediate compartment,Host Golgi apparatus,host perinuclear region
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.