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Histone H1.4 Rabbit mAb [6L2L]Cat NO.: A36612

Western blot(SDS PAGE) analysis of extracts from HCT116 cells.Using Histone H1.4 Rabbit mAb [6L2L] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :H1-4,H1F4,HIST1H1E,H14_HUMAN,Histone H1.4

UniProtID :P10412

MASS(da) :21,865

MW(kDa) :30 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Histone H1.4

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HCT116 cells

Function : Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity)..

Subcellular locationi :Nucleus. Chromosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.