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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using PFKFB3 Rabbit mAb [9MPE] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PFKFB3,F263_HUMAN,6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 [Includes: 6-phosphofructo-2-kinase ,Fructose-2,6-bisphosphatase ]
UniProtID :Q16875
MASS(da) :59,609
MW(kDa) :60kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit monoclonal IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PFKFB3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes both the synthesis and degradation of fructose 2,6-bisphosphate..
Tissue specificity :Ubiquitous.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.