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Western blot(SDS PAGE) analysis of extracts from C6 cells.Using βII-Tubulin Rabbit mAb [01J1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TUBB2A,TUBB2,TBB2A_HUMAN,Tubulin beta-2A chain
UniProtID :Q13885
MASS(da) :49,907
MW(kDa) :50KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit monoclonal IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human βII-Tubulin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :C6 cells
Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity)..
Tissue specificity :High expression in brain, where it represents 30% of all beta-tubulins..
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.