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βII-Tubulin Rabbit mAb [01J1]Cat NO.: A63766

Western blot(SDS PAGE) analysis of extracts from C6 cells.Using βII-Tubulin Rabbit mAb [01J1] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TUBB2A,TUBB2,TBB2A_HUMAN,Tubulin beta-2A chain

UniProtID :Q13885

MASS(da) :49,907

MW(kDa) :50KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit monoclonal IgG

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human βII-Tubulin.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :C6 cells

Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity)..

Tissue specificity :High expression in brain, where it represents 30% of all beta-tubulins..

Subcellular locationi :Cytoplasm, cytoskeleton.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.