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Western blot(SDS PAGE) analysis of extracts from Raji cells.Using p47phox Rabbit mAb [1Y81] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NCF1,NOXO2,SH3PXD1A,NCF1_HUMAN,Neutrophil cytosol factor 1
UniProtID :P14598
MASS(da) :44,682
MW(kDa) : 47KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit monoclonal IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human p47phox.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raji cells
Function : NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production)..
Tissue specificity :Detected in peripheral blood monocytes and neutrophils (at protein level)..
Subcellular locationi :Cytoplasm, cytosol. Membrane,Peripheral membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.