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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using α-Actin-1 Rabbit mAb [31TE] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACTA1,ACTA,ACTS_HUMAN,Actin, alpha skeletal muscle [Cleaved into: Actin, alpha skeletal muscle, intermediate form]
UniProtID :P68133
MASS(da) :42,051
MW(kDa) :42kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit monoclonal IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human α-Actin-1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.