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Western blot(SDS PAGE) analysis of extracts from Recombinant SARS-CoV-2 Nucleoprotein.Using SARS-CoV-2 Nucleoprotein Rabbit mAb [P2RO] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SARS-CoV-2 Nucleoprotein ,Nucleoprotein
UniProtID :P0DTC9
MASS(da) :45,626
MW(kDa) : 50KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit monoclonal IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :SARS-CoV-2
Specificity :Antibody is produced by immunizing animals with a synthetic peptide ofSARS-CoV-2 Nucleoprotein.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Recombinant SARS-CoV-2 Nucleoprotein
Function :Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (By similarity). UniRule annotationBy similarity May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
Subcellular locationi :Host cytoplasm, Virion
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.