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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using ELK1 Rabbit mAb [9641] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ELK1,ELK1_HUMAN,ETS domain-containing protein Elk-1
UniProtID :P19419
MASS(da) :44,888
MW(kDa) :62kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human ELK1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells.
Function : Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity)..
Tissue specificity :Lung and testis.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.