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Western blot analysis of extracts from Human testis tissue lyaste.using PGK1 + PGK2 Rabbit mAb [52B3] at dilution of 1:1000 incubated at 4℃ over night
Protein names :PGK2,PGKB,PGK2_HUMAN,Phosphoglycerate kinase 2
UniProtID :P00558,P07205
MASS(da) :44,796
MW(kDa) :45 kda
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human PGK1 + PGK2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human testis tissue lyaste
Function : Essential for sperm motility and male fertility (PubMed:26677959). Not required for the completion of spermatogenesis (By similarity)..
Tissue specificity :Mainly found in round spermatids. Localized on the principle piece in the sperm (at protein level). Testis-specific. Expression significantly decreased in the testis of elderly men..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.