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c-Jun (S63) Rabbit mAb [744R]Cat NO.: A12004

Western blot analysis of extracts from HeLa cells lysates anisomycin-treated .using c-Jun (S63) Rabbit mAb [744R] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :JUN,JUN_HUMAN,Transcription factor Jun

UniProtID :P05412

MASS(da) :35,676

MW(kDa) :42 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • 免疫荧光(ICC/IF)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser63 of Human c-Jun .

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells lysates anisomycin-treated

Tissue specificity :Expressed in the developing and adult prostate and prostate cancer cells..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.