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Western blot analysis of extracts from HeLa cells lysates treated with FBS+CA .using Cytokeratin 8 (S431) Rabbit mAb [85L0] at dilution of 1:1000 incubated at 4℃ over night
Protein names :KRT8,CYK8,K2C8_HUMAN,Keratin, type II cytoskeletal 8
UniProtID :P05787
MASS(da) :53,704
MW(kDa) :52 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser431 of Human Cytokeratin 8 .
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells lysates treated with FBS+CA
Function : Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle..
Tissue specificity :Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity..
Subcellular locationi :Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.