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Cytokeratin 8 (S431) Rabbit mAb [85L0]Cat NO.: A89081

Western blot analysis of extracts from HeLa cells lysates treated with FBS+CA .using Cytokeratin 8 (S431) Rabbit mAb [85L0] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :KRT8,CYK8,K2C8_HUMAN,Keratin, type II cytoskeletal 8

UniProtID :P05787

MASS(da) :53,704

MW(kDa) :52 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • 免疫荧光(ICC/IF)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser431 of Human Cytokeratin 8 .

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells lysates treated with FBS+CA

Function : Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle..

Tissue specificity :Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity..

Subcellular locationi :Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.