HIF-2α Rabbit mAb [F11W]Cat NO.: A34650

Protein names ：EPAS1,BHLHE73,HIF2A,MOP2,PASD2,EPAS1_HUMAN,Endothelial PAS domain-containing protein 1

UniProtID ：Q99814

MASS(da) ：96,459

MW(kDa) ：120 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human HIF-2α

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：A498 cells

Function ： Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT,heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity)..

Tissue specificity ：Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Subcellular locationi ：Nucleus. Nucleus speckle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.