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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using HIF-2α Rabbit mAb [UJU7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EPAS1,BHLHE73,HIF2A,MOP2,PASD2,EPAS1_HUMAN,Endothelial PAS domain-containing protein 1
UniProtID :Q99814
MASS(da) :96,459
MW(kDa) :120 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human HIF-2α
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT,heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity)..
Tissue specificity :Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
Subcellular locationi :Nucleus. Nucleus speckle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.