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Western blot analysis of extracts from SH-SY5Y cells lyastes.using GAP43 Rabbit mAb [DX09] at dilution of 1:1000 incubated at 4℃ over night
Protein names :GAP43,NEUM_HUMAN,Neuromodulin
UniProtID :P17677
MASS(da) :24,803
MW(kDa) :48 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human GAP43.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cells lyastes
Function : This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction..
Subcellular locationi :Cell membrane,Peripheral membrane protein,Cytoplasmic side. Cell projection, growth cone membrane,Peripheral membrane protein,Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane,Peripheral membrane protein. Perikaryon. Cell projection, dendrite. Cell projection, axon. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.