PLC-gamma-1 (Y783) Rabbit mAb [2NQC]Cat NO.: A74937

Protein names ：PLCG1,PLC1,PLCG1_HUMAN,1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

UniProtID ：P19174

MASS(da) ：148,532

MW(kDa) ：149 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫荧光(ICC/IF)1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr783 of Human PLC-gamma-1 .

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：Jurkat cells lysates Pervanadate-treated

Function ： Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Plays a role in actin reorganization and cell migration (PubMed:17229814)..

Subcellular locationi ：Cell projection, lamellipodium. Cell projection, ruffle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.