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AGXT Rabbit mAb [4571]Cat NO.: A82715

Western blot analysis of extracts from HepG2 cells lyastes.using AGXT Rabbit mAb [4571] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :AGXT,AGT1,SPAT,SPYA_HUMAN,Alanine--glyoxylate aminotransferase

UniProtID :P21549

MASS(da) :43,010

MW(kDa) :40 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human AGXT.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells lyastes

Function : Peroxisomal aminotransferase that catalyzes the transamination of glyoxylate to glycine and contributes to the glyoxylate detoxification (PubMed:10960483, PubMed:12777626, PubMed:24055001, PubMed:23229545, PubMed:26149463). Also catalyzes the transamination between L-serine and pyruvate and contributes to gluconeogenesis from the L-serine metabolism (PubMed:10347152)..

Tissue specificity :Liver.

Subcellular locationi :Peroxisome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.