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Cdc25A (S124) Rabbit mAb [5RQ5]Cat NO.: A42263

Western blot analysis of extracts from HeLa cells lyastes.using Cdc25A (S124) Rabbit mAb [5RQ5] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :CDC25A,MPIP1_HUMAN,M-phase inducer phosphatase 1

UniProtID :P30304

MASS(da) :59,087

MW(kDa) :59 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser124 of Human Cdc25A .

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells lyastes

Function : Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.