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Western blot analysis of extracts from A431 cells lyastes.using TRAP alpha/TRAPA Rabbit mAb [07G5] at dilution of 1:1000 incubated at 4℃ over night
Protein names :SSR1,TRAPA,PSEC0262,SSRA_HUMAN,Translocon-associated protein subunit alpha
UniProtID :P43307
MASS(da) :32,235
MW(kDa) :34 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human TRAP alpha/TRAPA.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cells lyastes
Function : TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.