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Western blot analysis of extracts from A431 cells lyastes.using ATP1B3 Rabbit mAb [CWZ6] at dilution of 1:1000 incubated at 4℃ over night
Protein names :ATP1B3,AT1B3_HUMAN,Sodium/potassium-transporting ATPase subunit beta-3
UniProtID :P54709
MASS(da) :31,513
MW(kDa) :40 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human ATP1B3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cells lyastes
Function : This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
Subcellular locationi :Apical cell membrane,Single-pass type II membrane protein. Basolateral cell membrane,Single-pass type II membrane protein. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.