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MetRS/MARS Rabbit mAb [81XQ]Cat NO.: A91541

Western blot analysis of extracts from HepG2 cells lyastes.using MetRS/MARS Rabbit mAb [81XQ] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :MARS1,MARS,SYMC_HUMAN,Methionine--tRNA ligase, cytoplasmic

UniProtID :P56192

MASS(da) :101,116

MW(kDa) :101 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human MetRS/MARS.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells lyastes

Function : Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:11714285). Plays a role in the synthesis of ribosomal RNA in the nucleolus (PubMed:10791971)..

Subcellular locationi :Cytoplasm, cytosol. Nucleus, nucleolus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.