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Western blot analysis of extracts from HepG2 cells lyastes.using MetRS/MARS Rabbit mAb [81XQ] at dilution of 1:1000 incubated at 4℃ over night
Protein names :MARS1,MARS,SYMC_HUMAN,Methionine--tRNA ligase, cytoplasmic
UniProtID :P56192
MASS(da) :101,116
MW(kDa) :101 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human MetRS/MARS.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells lyastes
Function : Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:11714285). Plays a role in the synthesis of ribosomal RNA in the nucleolus (PubMed:10791971)..
Subcellular locationi :Cytoplasm, cytosol. Nucleus, nucleolus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.