P-Histone H3 (S10) Rabbit mAb [1DRQ]Cat NO.: A13156

Protein names ：H3C1,H3FA,HIST1H3A,H3C2,H3FL,HIST1H3B,H3C3,H3FC,HIST1H3C,H3C4,H3FB,HIST1H3D,H3C6,H3FD,HIST1H3E,H3C7,H3FI,HIST1H3F,H3C8,H3FH,HIST1H3G,H3C10,H3FK,HIST1H3H,H3C11,H3FF,HIST1H3I,H3C12,H3FJ,HIST1H3J,H31_HUMAN,Histone H3.1

UniProtID ：P68431

MASS(da) ：15,404

MW(kDa) ：17 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Phospho-Histone H3 (Ser10)

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells treated with Nocodazole (100 ng/ml 16 hr)

Function ： Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subcellular locationi ：Nucleus. Chromosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.