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SMAD1+SMAD5+SMAD9 (S465+S467) Rabbit mAb [23PA]Cat NO.: A89781

Western blot analysis of extracts from HeLa cells lysates treated with BMP-4 .using SMAD1+SMAD5+SMAD9 (S465+S467) Rabbit mAb [23PA] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :SMAD9,MADH6,MADH9,SMAD8,SMAD9_HUMAN,Mothers against decapentaplegic homolog 9

UniProtID :Q15797,Q99717,O15198

MASS(da) :52,493

MW(kDa) :52 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser465 and Ser467 of Human SMAD1+SMAD5+SMAD9 .

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells lysates treated with BMP-4

Function : Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).

Tissue specificity :Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.

Subcellular locationi :Cytoplasm. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.