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Western blot analysis of extracts from SH-SY5Y cells lyastes.using αInternexin Rabbit mAb [M27Y] at dilution of 1:1000 incubated at 4℃ over night
Protein names :INA,NEF5,AINX_HUMAN,Alpha-internexin
UniProtID :Q16352
MASS(da) :55,391
MW(kDa) :55 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human αInternexin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cells lyastes
Function : Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (By similarity)..
Tissue specificity :Found predominantly in adult CNS.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.